The SCA® CASA System uses a combination of computerized algorithms and image-analysis software to accurately measure sperm motility, concentration, morphology, DNA fragmentation, vitality, acrosome reaction, and leukocytes. The system allows for the complete analysis of semen samples from a variety of species, including humans, animals, insects, fish, mice, and rats. The results are then compared to WHO 6 reference values to provide an objective assessment of the semen quality.
SCA® is a modular automatic system for the analysis of semen samples, according to pre-defined criteria for most vertebrates (mammals, avians, reptiles, amphibians, and fish) and invertebrates (e.g. echinoderms and molluscs).
Allows the accurate and objective analysis of a wide range of sperm kinematics, morphological, DNA and vitality parameters, as well as defining new analysis settings that adapt to new animal species or breeds.
The SCA® greatly improves the ability of the laboratory to accurately analyze motility sequences for motility analysis and sperm images for morphology, DNA fragmentation, vitality and acrosome reaction. Measurement errors due to human factors are minimized in the analysis of animal sperm.
SCA® system has been widely used in animal research, being versatile enough to suit a wide range of animal species, from invertebrates to mammals, including rodents and has even proven to be useful in the study of microalgae counting.
With a motorized stage fitted to the microscope the analysis process becomes fully automatic enabling the examination of 4 slides or counting chambers consecutively, without the presence of the operator.
Ideal for testing various drugs, chemicals and radiations for their toxicity. This system adheres to 21 CFR11 guidelines.
The laptop configuration allows the SCA® to be transported almost anywhere in the field, enabling e.g. animal fertility studies in situ, during the breeding season.
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| MOTILITY AND CONCENTRATION |
MORPHOLOGY | DNA FRAGMENTATION |
VITALITY | ACROSOME REACTION |
| Analysis of the motility, concentration and kinematic parameters | Analysis of the morphology and morphometry in stained sperm samples | Analysis of the DNA fragmentation with the chromatin dispersion test | Analysis of vitality using fluorescence microscopy | Analysis of intact and acrosome-reacted sperm using fluorescence |
Automatic analysis of hyperactive and mucus penetration, information about the capacity of sperm to fertilize the egg.
Can be used to modify parameters for existing sperm functional tests or create new cut-off points for rapid sperm, progressiveness, etc.
The SCA system works with touch screens, multi- screens, leap motion and portable devices.
Fully customizable.
Automatic analysis of intact and acrosome-reacted sperm with FluoAcro kit
Enable the operator to amalgamate any of the database results into subgroups and create customized statistics.
Installation, training and accreditation by specialists.
More than 40 different reports with the possibility of selecting images and graphics (including the Levy-Jennings report for quality control).
SCA® offers several innovations in automatic analysis such as: fluorescence analysis of sperm concentration, motility, and DNA fragmentation; very low concentration sample analysis; and tail detection in morphology.
SCA® is ready to connect with existing databases.
| SCA® Motility and Concentration, Droplets |
SCA® Morphology |
SCA® DNA Fragmentation |
SCA® Vitality |
SCA® Acrosome Reaction |
SCA® Acrosome Integrity |
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| COMPUTER | Desktop or laptop: Operating system Windows 10 (64 bits); Processor: Intel Core i5 or superior; RAM: 4 GB or superior; USB 3.0 port |
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| CAMERA | Basler Ace acA1300-200uc (High resolution and up to 200 fps) | |||||
| MICROSCOPE | Nikon or Olympus; Trinocular tube 1x, C-mount 1x; Turret condenser and centering telescope | |||||
| OBSERVATION METHOD |
Negative phase contrast |
Fluorescence | Brightfield | Brightfield | Fluorescence | Fluorescence | Fluorescence | Phase contrast |
| OBJECTIVE | 10x Ph- | 40x / 60x | 10x | 20x | 40x Plan Fluor | 40x ph+ | ||
| FILTERS |
Green | Long bandpass (EX 330- 380; EM 420; DM 400) |
Blue | Green | Bandpass (EX 510- 560; EM 590; DM 565) |
Long bandpass (EX 330-380; EM 420; DM 440) |
Dual band (DAPI/FITC) (EX 387/480, EM 433/530, DM 403/502) |
Green |
| DISPOSABLES | Disposable counting chambers or |
SpermBlue | Chromatin dispersion | FluoVit | FluoAcro | – | ||
| KITS, STAININGS |
Makler | test kits (SCD) | ||||||
| – | FluoCount | – | – | Heating stage | – | – | ||
Automatic measuring system for the analysis of semen samples, following WHO criteria or others.
Many scientific papers have revealed the homogeneous results obtained with CASA2, in contrast to the often unreliable outcome from conventional analysis.
SCA® will assist in the implementation of the objective analysis and quality controls in your laboratory. In addition, it produces all of the information needed for the norms ISO 15189:2013 and ISO 23162:2021 accreditations.
The SCA® greatly improves the ability of the laboratory to compete through the consistency of the analysis by recording image sequences and results. Measurement errors due to human factors are minimized in the analysis of human sperm.
Various studies have shown that the SCA® provides reliable, linear and accurate results with less variability than manual methods, giving higher predictive values when diagnosing fertility problems.
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| MOTILITY AND CONCENTRATION |
MORPHOLOGY | DNA FRAGMENTATION |
VITALITY | ACROSOME REACTION |
LEUKOCYTES |
| Analysis of the motility, concentration and kinematic parameters | Analysis of the morphology and morphometry in stained sperm samples | Analysis of the DNA fragmentation with the chromatin dispersion test | Analysis of the vitality under brightfield or fluorescence | Analysis of intact and acrosome reacted under fluorescence | Analysis of human peroxidase-positive leukocytes |
We developed BrightVit, a new kit to stain in one step to assess quickly the vitality in brightfield (possible to see the HOS). SCA® Vitality can automatically analyze in brightfield or fluorescence.
Automatic analysis of hyperactive and mucus penetration, information about sperm capacity to fertilize the egg.
Automatically provides a diagnosis and recommends the best-assisted reproduction treatment to follow.
Enable the operator to amalgamate any of the database results into subgroups and create customised statistics.
Working with touch screens, multi-screens, leap motion and portable devices.
Automatic detection of peroxidase-positive cells in semen.
Automatic analysis of intact and acrosome-reacted sperm with FluoAcro kit.
SCA® offers several innovations in automatic analysis such as: an intelligent filter for sperm detection; fluorescence analysis for sperm concentration, motility and DNA fragmentation; very low concentration samples analysis; and vacuoles and tails detection in morphology.
With a motorized stage, the analysis process is fully automatic enabling the examination of 4 slides, without user intervention.
SCA® is ready to connect with existing databases.
| OBSERVATION METHOD |
Negative phase contrast |
Fluorescence | Brightfield | Brightfield | Fluorescence | Fluorescence | Fluorescence | Brightfield |
| OBJECTIVE | 10x Ph- | 40x / 60x | 10x | 20x | 40x Plan Fluor | 40x | ||
| FILTERS |
Green | Long bandpass (EX 330-380;EM 420; DM 400) |
Blue | Green | Bandpass (EX 510- 560; EM 590; DM 565) |
Long bandpass (EX 330-380; EM 420; DM 440) |
Dual band (DAPI/FITC) (EX 387/480, EM 433/530, DM 403/502) |
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| DISPOSABLES, KITS, STAININGS |
Disposable counting chambers or Makler |
SpermBlue | Chromatin dispersion test kits (SCD) |
FluoVit | FluoAcro | Leukocytes slides SCA | ||
| DISPOSABLES, KITS, STAININGS |
– | FluoCount | – | – | Heating stage | – | – | |
SCA® is a modular automatic system for research and allied applications. CASA Sperm Class Analyzer is the most advanced and the best system for research.
Ideal for testing drugs, chemicals and radiations for their toxicity.
Analysis of sperm motility and sperm count.
Morphometry analysis and morphology classification of stained semen samples observed in brightfield microscopy.
Analysis for evaluating the level of damage in the sperm chromatin. Moderate damage is related to alterations during embryo and fetal development.
Estimated by assessing the membrane integrity of the cells.
It provides the percentage of acrosome intact and acrosome-reacted spermatozoa.